Ijprbs 296

This study was designed to determine the antioxidant and hepatoprotective effect of nbutanol soluble fractions of Albizzia lebbeck The various extracts were prepared and extracts containing flavanoids, tannins and saponins (Methanolic extract) was selected for isolation of chemical constituents. nbutanol soluble fractions of extract (BFALB) and leaf extract (BFALL) were selected for screening of vitro antioxidant activity such as reducing power, superoxide anion and hydroxyl radical scavenging activity. Both fractions were subjected to acute toxicity study. BFALB and BFALL were also screened for its influence on tissue GSH levels and lipid peroxidation (In-vivo antioxidant models). The 60 mg/Kg BFALB and 20 mg/Kg BFALL were further screened for hepatoprotective (against CCl4 induced hepatotoxicity) activities. The various relevant biochemical markers like SGOT, SGPT, ALP, Total Bilirubin, Direct Bilirubin were estimated to assess the hepatoprotective potential of the fractions. BFALB and BFALL demonstrated dose dependant activities in all the models of the study. The treatments with fractions have reversed all the biochemical markers of hepatotoxicity to the near normal levels. From the results it may be concluded that the fractions of Albizzia lebbeck bark and leaf possess antioxidant and hepatoprotective activity. The antioxidant and hepatoprotective activity of the plant may be attributed to the polyphenolic compounds like saponins that are present in the plant. ISSN: 2277-8713 IJPRBS